February 22, 2017

Advances in Forensic Haemogenetics: 14th Congress of the by Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M.

By Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (auth.), Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (eds.)

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Extra resources for Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991

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5%. The repeat length of 16 bp was readily apparent from the difference in size between adjacent bands. , 1990). 8%. This degree of accuracy was typical for ali allele sizes found in the sample set. A higher degree of accuracy would be expected with a D1S80 allelic size ladder, since DNA fragments composed of specific sequence repeats should migrate differently than restriction fragments composed of a random base composition. 37 Since the DNA fragments run off the gel during electrophoresis, the gel may be re-used for further separations.

One possible improvement would be to labei the "COL 2A1" fragments radioactively and to separate them on a sequencing gel (40 cm PAG, denaturing conditions). Sequencing of the individual alleles to verify the gene model would also be desirable. The "COL 2A1" system seems to be much more complex than was originally assumed in previous publications (Stoker el al. 1985; Priestley el al. 1990; Wu el al. 1990). Nevertheless this system can be a useful tool for identification and paternity analysis using a binning approach.

Thus, bands of different color, representing different alleles, appear as individual entities. The sizes of the sample bands are then automatically determined from the calibration curve obtained from the co-electrophoresed standard size ladder. We used a second order least squares curve fit to the size ladder to obtain the calibration curve. The analyzed data are stored in a tabular form suitable for export to database management software. , 1989), and COL 2A1 (Wu and Beii, 1990) using published sequences for the primers, generally using 20 ng DNA in the PCR.

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